mkn45 human gc cell lines  (Procell Inc)

Journal: Oncology Reports

Article Title: NR4A1 mediates chemotherapy-induced senescence via the PI3K/AKT pathway in gastric cancer cells

doi: 10.3892/or.2026.9080

Figure Lengend Snippet: OXA induces senescence was verified in gastric cancer cells. (A and B) SA-β-Gal staining of Ctrl and OXA groups and quantification of SA-β-Gal positive cells in AGS and MKN45 cells. (C) p21 protein expression and quantification. (D and E) Immunofluorescence of γ-H2AX and quantification. Red fluorescence indicates γ-H2AX staining, and blue fluorescence reflects nuclear staining with DAPI. (F) mRNA levels of the senescence-associated secretory phenotype factors. (G) CDK4, cyclin D1, RB, p-RB protein expression, and quantification in the two groups. Data represent the mean ± SD of at least three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. OXA, oxaliplatin; SA-β-Gal, senescence-associated β-galactosidase; p-, phosphorylated.

Article Snippet: AGS and MKN45 human GC cell lines were acquired from the Procell Life Science & Technology Co., Ltd. and maintained in RPMI-1640 containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C with 5% CO 2 condition.

Techniques: Staining, Expressing, Immunofluorescence, Fluorescence

Journal: Nature Communications

Article Title: Multitrait GWAS and functional validation reveal genetic loci for gastric cancer

doi: 10.1038/s41467-026-70774-9

Figure Lengend Snippet: A mRNA levels were measured by RT‑qPCR in AGS and MKN45 cells transfected with control (CON) or gene‑specific overexpression plasmids for NDUFA12 , FGD6 , NR2C1 or VEZT at 48 h. Data are presented as the mean ± SD from n = 3 biologically independent experiments. Significance relative to the CON for each gene was assessed using an unpaired two‑tailed t ‑test (no adjustment for multiple comparisons) and denoted as * P < 0.05, ** P < 0.01 and *** P < 0.001; ns not significant. Exact P values are provided in the figure. B Cell viability was monitored over time. Data are presented as the mean ± SD from n = 3 biologically independent experiments, each performed in triplicate wells. Significance at each time point relative to CON was determined by two‑way ANOVA followed by Tukey’s multiple comparisons test and denoted as ns not significant, * P < 0.05, ** P < 0.01 and *** P < 0.001. Exact P values are provided in the figure. C , D EdU staining ( C ) and colony formation assays ( D ) were performed in transfected AGS and MKN45 cells. Images are representative of n = 3 biologically independent experiments. Scale bar, 100 μm. CON control, OP overexpression.

Article Snippet: The HEK293T, AGS and MKN45 cell lines were obtained from Procell (Wuhan, China) and cultured in DMEM (for HEK293T), Ham’s F-12K (for AGS) or RPMI-1640 (for MKN45) medium (all from Gibco, Carlsbad, CA, USA), each supplemented with 10% foetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: Transfection, Control, Over Expression, Staining

Journal: Nature Communications

Article Title: Multitrait GWAS and functional validation reveal genetic loci for gastric cancer

doi: 10.1038/s41467-026-70774-9

Figure Lengend Snippet: Wound-healing ( A ; scale bar, 250 μm) and Transwell migration ( B ; scale bar, 100 μm) assays were performed using AGS and MKN45 cells transfected with control (CON) or gene‑specific overexpression (OP) plasmids. Images are representative of three biologically independent experiments with consistent results. CON control, OP overexpression.

Article Snippet: The HEK293T, AGS and MKN45 cell lines were obtained from Procell (Wuhan, China) and cultured in DMEM (for HEK293T), Ham’s F-12K (for AGS) or RPMI-1640 (for MKN45) medium (all from Gibco, Carlsbad, CA, USA), each supplemented with 10% foetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: Migration, Transfection, Control, Over Expression

Journal: BMC Cancer

Article Title: β-ionone synergizes with 5-Fluorouracil to inhibit gastric cancer progression through PAX6-mediated cell cycle arrest

doi: 10.1186/s12885-026-15540-2

Figure Lengend Snippet: BI and 5-FU exerted independent antiproliferative effects on gastric cancer cells. A MTT assay was used to detect the effects of BI and 5-FU at different concentrations and time on the viability of MKN45 cells, AGS cells, and NCI-N87 cells ( n = 5); ( B-C ) MTT assay was used to detect the effects of BI, 5-FU or their combination at different concentrations for 72 h on the viability of MKN45 cells and AGS cells and the synergistic effect of combination treatment was estimated by calculation of CI index using Compusyn software; ( D ) MTT assay was used to detect the effects of BI (144 µmol/L) and 5-FU (0.22 µmol/L) for 72 h on the viability of GES-1 cells and HS5 cells ( n = 5). Data are presented as mean ± S.D. of triplicates. Statistical analyses were performed using one-way ANOVA. Compared with the control group, * P < 0.05, ** P < 0.01. ns, not significant

Article Snippet: The gastric adenocarcinoma cell lines - MKN45 cells, AGS cells, NCI-N87 cells and human normal gastric gland GES-1 cells were purchased from Wuhan Procell Life Technology Co.,Ltd. (Wuhan, China).

Techniques: MTT Assay, Software, Control

Journal: BMC Cancer

Article Title: β-ionone synergizes with 5-Fluorouracil to inhibit gastric cancer progression through PAX6-mediated cell cycle arrest

doi: 10.1186/s12885-026-15540-2

Figure Lengend Snippet: BI and 5-FU affected DNA synthesis, cell cycle, and stem cell characteristics in gastric cancer cells. MKN45 cells and AGS cells were harvested after being treatmented with BI (144 µmol/L and 80 µmol/L) and 5-FU (0.22 µmol/L and 7 µmol/L) alone or in combination for 72 h. A DNA synthesis in MKN45 cells and AGS cells was detected by flow cytometry following Edu addition and staining ( n = 3); ( B ) The cell cycle in MKN45 cells and AGS cells was detected by flow cytometry after PI staining ( n = 3); ( C ) Effects of BI and 5-FU on the tumor sphere-forming ability of MKN45 CSCs. The number of tumor spheres was counted under an optical microscope (40×, scale bar: 50 μm). Data are presented as mean ± S.D. of triplicates. Statistical analyses were performed using one-way ANOVA. Compared with the control group, * P < 0.05, ** P < 0.01. Compared with the combination of BI with 5-FU, # P < 0.05,## P < 0.01

Article Snippet: The gastric adenocarcinoma cell lines - MKN45 cells, AGS cells, NCI-N87 cells and human normal gastric gland GES-1 cells were purchased from Wuhan Procell Life Technology Co.,Ltd. (Wuhan, China).

Techniques: DNA Synthesis, Flow Cytometry, Staining, Microscopy, Control

Journal: BMC Cancer

Article Title: β-ionone synergizes with 5-Fluorouracil to inhibit gastric cancer progression through PAX6-mediated cell cycle arrest

doi: 10.1186/s12885-026-15540-2

Figure Lengend Snippet: BI and 5-FU affected the expression of PAX6, GSK-3beta, E2F1, and proteins related to the cell cycle in MKN45 cells. MKN45 cells treated with BI, 5-FU, or their combination for 72 h, the expression of PCNA ( A ), E2F1, Cyclin D1, CDK4 ( B ), and PAX6 ( E ) was determined by Western blot ( n = 3); ( C ) The TCGA data for gastric adenocarcinoma and normal tissue data for GTEx, the statistical method was used the Wilcoxon rank sum test and visualized the data using the ggplot2 package; ( D ) The correlation between total survival time of gastric cancer patients and PAX6 in the kmplot database; ( F ) The expression of PAX6 protein in MKN45 cells by immunofluorescence assay (scale bar: 2 μm). Data are presented as mean ± S.D. of triplicates. Statistical analyses were performed using one-way ANOVA, unpaired Student’s t-tests and Log-rank test. Compared with the control group, * P < 0.05, ** P < 0.01. Compared with the combination of BI with 5-FU, # P < 0.05,## P < 0.01

Article Snippet: The gastric adenocarcinoma cell lines - MKN45 cells, AGS cells, NCI-N87 cells and human normal gastric gland GES-1 cells were purchased from Wuhan Procell Life Technology Co.,Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot, Immunofluorescence, Control

Journal: BMC Cancer

Article Title: β-ionone synergizes with 5-Fluorouracil to inhibit gastric cancer progression through PAX6-mediated cell cycle arrest

doi: 10.1186/s12885-026-15540-2

Figure Lengend Snippet: BI and 5-FU affected the expression of proteins related to PAX6/GSK-3β pathways in MKN45 cells. The expression of GSK-3β and PAX6 in MKN45 cells treated with BI, 5-FU, or their combination with an inhibitor of GSK-3β - CHIR-99,021 for 72 h was determined by Western blot and IP. A The expression of pGSK-3β ( n = 3); ( B ) IP assay was used to determine the interaction of PAX6 and GSK-3β; ( C ) The expression of GSK-3β and PAX6 in MKN45 cells after siRNA knockdown ( n = 3); ( D ) IP assay was used to determine that after pre-treatment with CHIR-99,021(7 nmol/L) for 24 h, the interaction of PAX6 and GSK-3β were weakened interaction; ( E ) The expression of PAX6 and GSK-3β was determined in MKN45 cells ( n = 3); ( F ) The cell cycle was detected in MKN45 cells by flow cytometry after PI staining ( n = 3). Data are presented as mean ± S.D. of triplicates. Statistical analyses were performed using one-way ANOVA. Compared with the control group, * P < 0.05, ** P < 0.01. Compared with the combination of BI with 5-FU, # P < 0.05,## P < 0.01.IB: Immumoblotting; IP: Immumoprecipitation

Article Snippet: The gastric adenocarcinoma cell lines - MKN45 cells, AGS cells, NCI-N87 cells and human normal gastric gland GES-1 cells were purchased from Wuhan Procell Life Technology Co.,Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot, Knockdown, Flow Cytometry, Staining, Control

Journal: BMC Cancer

Article Title: β-ionone synergizes with 5-Fluorouracil to inhibit gastric cancer progression through PAX6-mediated cell cycle arrest

doi: 10.1186/s12885-026-15540-2

Figure Lengend Snippet: BI and 5-FU inhibited the growth of MKN45 cell xenografts in a nude mouse model. A BI and 5-FU administration schedule for the nude mice experiment ( n = 5); ( B ) Changes in body weight of nude mice in each group during the administration ( n = 5); ( C ) Changes in tumor volume of nude mice during the administration in each group ( n = 5); ( D ) Images of the tumors dissected from the animals; ( E ) Tumor weight of nude mice in each group at the termination of experiments ( n = 5); ( F ) Tumor volume of nude mice in each group at the termination of experiments ( n = 5); ( G ) Morphological changes of MKN45 cell xenografts by HE staining analysis (scale bar: 50 μm). Data are presented as mean ± S.D. of triplicates. Statistical analyses were performed using one-way ANOVA. Compared with the control group, * P < 0.05, ** P < 0.01. Compared with the combination of BI with 5-FU, # P < 0.05,## P < 0.01

Article Snippet: The gastric adenocarcinoma cell lines - MKN45 cells, AGS cells, NCI-N87 cells and human normal gastric gland GES-1 cells were purchased from Wuhan Procell Life Technology Co.,Ltd. (Wuhan, China).

Techniques: Staining, Control

Journal: BMC Cancer

Article Title: β-ionone synergizes with 5-Fluorouracil to inhibit gastric cancer progression through PAX6-mediated cell cycle arrest

doi: 10.1186/s12885-026-15540-2

Figure Lengend Snippet: The apoptotic determination and the expression of Cleaved-caspase-3, PCNA, and PAX6 in MKN45 cell xenografts. A The number of apoptotic cells in xenografts was detected by TUNEL; ( B , C , D ) The expression of Cleaved-caspase-3, PCNA, and PAX6 proteins in MKN45 cell xenografts was detected by IHC ( n = 5) (scale bar: 100 μm, 50 μm). Data are presented as mean ± S.D. of triplicates. Statistical analyses were performed using one-way ANOVA. Compared with the control group, * P < 0.05, ** P < 0.01. Compared with the combination of BI with 5-FU, # P < 0.05,## P < 0.01

Article Snippet: The gastric adenocarcinoma cell lines - MKN45 cells, AGS cells, NCI-N87 cells and human normal gastric gland GES-1 cells were purchased from Wuhan Procell Life Technology Co.,Ltd. (Wuhan, China).

Techniques: Expressing, TUNEL Assay, Control

Journal: BMC Cancer

Article Title: β-ionone synergizes with 5-Fluorouracil to inhibit gastric cancer progression through PAX6-mediated cell cycle arrest

doi: 10.1186/s12885-026-15540-2

Figure Lengend Snippet: The expression of PCNA, PAX6, GSK-3β, and proteins related to the cell cycle in MKN45 cell xenografts. The expression of PCNA ( A ), E2F1, Cyclin D1, CDK4 ( B ), PAX6 ( C ), and GSK-3β ( D ) in MKN45 cell xenografts ( n = 3); ( E ) Mechanistic diagram of BI increasing the sensitivity of gastric cancer cells to 5-FU. Statistical analyses were performed using one-way ANOVA. Compared with the control group, * P < 0.05, ** P < 0.01. Compared with the combination of BI with 5-FU, # P < 0.05,## P < 0.01

Article Snippet: The gastric adenocarcinoma cell lines - MKN45 cells, AGS cells, NCI-N87 cells and human normal gastric gland GES-1 cells were purchased from Wuhan Procell Life Technology Co.,Ltd. (Wuhan, China).

Techniques: Expressing, Control